Open AccessIdentification of Papillomaviral DNA Sequences in Hairless Mouse Tumours Induced by Ultraviolet Irradiation
Author(s) -
Peta A. Tilbrook,
Gavin E. Greenoak,
Vivienne E. Reeve,
P. J. Canfield,
Lutz Gissmann,
C. H. Gallagher,
Jerzy K. Kulski
Publication year - 1989
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-70-4-1005
Subject(s) - hairless , biology , virology , dna , identification (biology) , ultraviolet irradiation , ultraviolet , microbiology and biotechnology , irradiation , genetics , botany , quantum mechanics , nuclear physics , physics
Papillomas, carcinomas in situ and squamous cell carcinomas were induced using ultraviolet irradiation in the hairless mouse strain Mus musculus HRA/Skh. DNA extracted from biopsies was examined using Mastomys natalensis papillomaviral DNA as a hybridization probe at reduced stringency. Sequences homologous to the probe were detected in 16 of 24 papillomas, five of five carcinomas in situ and six of 38 squamous cell carcinomas. A number of tumour DNAs (16/33) also hybridized with mixed DNAs of human papillomavirus types 11, 13, 16 and 18 at reduced stringency. This suggests a role for the hairless mouse as a laboratory model for the study of the involvement of papillomaviruses in malignant transformations.