Open AccessGeneration of a Herpes Simplex Virus Type 1 Variant Devoid of XbaI Sites
Author(s) -
MacLean Ar,
Brown Sm
Publication year - 1987
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-68-4-1165
Subject(s) - biology , herpes simplex virus , gene , mutagenesis , virus , virology , coding region , genetics , thymidine kinase , genome , glycoprotein , phenotype , microbiology and biotechnology , mutation
Using both selection enrichment and site-directed mutagenesis, a herpes simplex virus type 1 (HSV-1) strain 17 genome lacking all four XbaI sites has been generated. The site at 0.45 map units which lies within the gene coding for a polypeptide of 28,000 molecular weight was removed by selection enrichment, while the site at 0.29 map units which lies within the gene coding for glycoprotein H was removed by site-directed mutagenesis. The parental virus from which these two XbaI sites were deleted had previously had the sites at 0.07 and 0.63 map units removed through selection enrichment. The variant devoid of XbaI sites (X4) showed normal growth characteristics; its phenotype was normal apart from the absence of the thymidine kinase protein, which is believed to be unrelated to the loss of XbaI sites.