Open AccessNucleotide Sequence of cDNA Clones Encoding the Outer Capsid Protein, VP5, of Bluetongue Virus Serotype 10
Author(s) -
Purdy Ma,
Ritter Gd,
Polly Roy
Publication year - 1986
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-67-5-957
Subject(s) - capsid , biology , orbivirus , complementary dna , virology , open reading frame , rna , microbiology and biotechnology , nucleic acid sequence , virus , peptide sequence , dna , gene , genetics , reoviridae , rotavirus
The complete sequence of the RNA segment that codes for a major outer capsid protein (VP5) of bluetongue virus serotype 10 has been determined from overlapping cDNA clones inserted into pBR322. The segment 5 RNA of the virus (M5 RNA) is deduced to be 1638 base pairs long (1.05 X 10(6) daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 59 163 daltons (526 amino acids) and a net charge of -4.5 at neutral pH.