Open AccessCharacterization of Ribonucleic Acid Transcriptase in Ibaraki Virus Core Particles
Author(s) -
Mikie Namiki,
Yujiro Suzuki,
Susumu Nakagawa,
Isao Yoshioka,
Y Saito
Publication year - 1983
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-64-6-1397
Subject(s) - rna , reverse transcriptase , biology , virology , virus , microbiology and biotechnology , rna directed dna polymerase , biochemistry , gene
Ibaraki virus core particles were purified from infected BHK-21 cells. The core particles displayed RNA transcriptase activity while the virion did not. The RNA transcriptase activity was stimulated about 15-fold by addition of S-adenosyl-L-methionine. The reaction product was single-stranded RNA which could be hybridized with heat-denatured Ibaraki virus RNA. The hybrids possessed the same electrophoretic mobility as Ibaraki virus double-stranded RNA.