Open AccessFactors Affecting In vitro DNA Ejection of the Lactobacillus lactis Bacteriophage LL-H
Author(s) -
Tapani Alatossava
Publication year - 1982
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-59-1-173
Subject(s) - tris , bacteriophage , hydroxymethyl , divalent , biology , in vitro , dna , lytic cycle , lactobacillus , microbiology and biotechnology , virology , biochemistry , bacteria , escherichia coli , stereochemistry , virus , chemistry , organic chemistry , genetics , gene
Tris(hydroxymethyl)aminomethane (tris) inactivates Lactobacillus lactis bacteriophages LL-H in vitro. The inactivation is caused by DNA ejection. This effect occurs in tris-HCl buffer only. Both pH and temperature affect the sensitivity of the phage to the tris treatment. The divalent cation Mg2+ prevented the inactivating effected of tris at concentrations about 10(3)-fold lower than monovalent cation K+.