Open AccessPolypeptide Synthesis Catalysed by Components of Pichinde Virus Disrupted by Detergent
Author(s) -
D N Chinault,
Herbert A. Thompson,
J. David Gangemi
Publication year - 1981
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-55-1-213
Subject(s) - ribosome , centrifugation , biology , protein biosynthesis , translation (biology) , messenger rna , gtp' , polysome , virus , ribosomal rna , biochemistry , rna , virology , enzyme , gene
Pichinde virus preparations were investigated for ribosomal components and associated activities. After detergent treatment and fractionation, viral components were assayed for polypeptide synthesis, elongation of nascent polypeptide chains, and mRNA activity. It was demonstrated that these subviral particles could synthesize polypeptides when exogenous mRNA template, aminoacyl--tRNAs, translation factors, GTP and appropriate cations were added. Undisrupted, whole virions could not synthesize polypeptide; disruption and subsequent centrifugation of subviral ribosomes was a prerequisite for the biosynthetic activity described.