Open AccessElectrophoretic Separation of Influenza Virus Ribonucleoproteins
Author(s) -
P. J. Rees,
Nigel J. Dimmock
Publication year - 1981
Publication title -
journal of general virology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-53-1-125
Subject(s) - ribonucleoprotein , biology , rna , ribonucleoprotein particle , virology , virus , electrophoresis , polyacrylamide gel electrophoresis , influenza a virus , genetics , biochemistry , gene , enzyme
A new procedure for the separation of influenza virus particle ribonucleoproteins (RNPs) by electrophoresis on a slab of polyacrylamide gel resolves five discrete bands. The largest is a triplet containing the three largest RNAs (1 to 3), the intermediate sized RNP is a doublet containing RNAs 5 and 6 and the others each contain a single RNA. In addition, each RNP is composed of NP protein and an amount of M which is independent of the size of the RNP. This suggests that some virus particle M is specifically associated with influenza ribonucleoprotein.