Open AccessLack of Correlation between pp60src Kinase Activity and Transformation Parameters in Cells Infected with Temperature-conditional Mutants of Rous Sarcoma Virus
Author(s) -
Michael J. Weber,
Helga Rübsamen,
Robert R. Friis
Publication year - 1981
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-52-2-395
Subject(s) - rous sarcoma virus , proto oncogene tyrosine protein kinase src , biology , transformation (genetics) , mutant , virology , kinase , microbiology and biotechnology , virus , biochemistry , gene
The pp60src kinase activity of cells infected with temperature-conditional mutants of Rous sarcoma virus (RSV), which induce only a partial transformation, was compared to the pattern of transformation parameters induced by these mutants. The tsGI251-infected cells were thermosensitive for hexose transport, fibrinolysis, morphological alterations and pp60src kinase activity, but cold-sensitive for loss of growth control. The tsGI253-infected cells were similar to tsGI251-infected cells except that they were only slightly cold-sensitive for loss of growth control and had a very low pp60src kinase activity which was temperature-insensitive. Thus, the pp60src kinase activity measured in vitro with IgG as a substrate did not correlate in a consistent way with any of the measured parameters of transformation.