Open AccessEvaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests
Author(s) -
T. Marrodan,
R. Nenova-Poliakova,
Manuel Rubio,
Javier Ariza,
Encarnación Clavijo,
Henk L. Smits,
Ramón Díaz
Publication year - 2001
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/0022-1317-50-8-663
Subject(s) - dipstick , dithiothreitol , antibody , brucella , immunoglobulin m , brucellosis , direct agglutination test , immunology , serology , brucellaceae , virology , medicine , chemistry , immunoglobulin g , microbiology and biotechnology , biology , urine , enzyme , brucella melitensis , biochemistry
The results of a dipstick assay for the detection of immunoglobulin M (IgM) to Brucella smooth lipopolysaccharide (S-LPS) correlated with those of an enzyme-linked immunosorbent assay (ELISA) for IgM and of the serum agglutination test (SAT) performed with and without dithiothreitol. Two sera which were dithiothreitol-sensitive and were dipstick negative were shown to contain specific IgA. The dipstick assay is recommended as a simple method for detecting specific IgM antibodies in acute-phase brucellosis patients.