Open AccessInvestigation of Immunoperoxidase-labelled Rotavirus in Tissue Culture by Light and Electron Microscopy
Author(s) -
D. Chasey
Publication year - 1980
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-50-1-195
Subject(s) - immunoperoxidase , fixative , electron microscope , biology , rotavirus , virology , ultrastructure , immunoelectron microscopy , virus , microscopy , pathology , cytoplasm , immunohistochemistry , antibody , biochemistry , immunology , anatomy , monoclonal antibody , physics , medicine , optics
A tissue culture-adapted strain of bovine rotavirus, grown in calf kidney monolayers, has been examined by light and electron microscopy after immunoperoxidase labelling. Some of the characteristic problems associated with pre-embedding methods have been demonstrated. Preparative techniques involving pretreatment of infected cells with a detergent followed by a detergent/fixative combination have enabled virus antigen to be labelled while maintaining satisfactory ultrastructural preservation.