Open AccessThe Characteristics of the Cell-free Translation of mRNA from Cells Infected with the Herpes Virus Pseudorabies Virus
Author(s) -
Bernadette M. McGrath,
William S. Stevely
Publication year - 1980
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-49-2-323
Subject(s) - pseudorabies , biology , virus , virology , messenger rna , reticulocyte , translation (biology) , in vitro , protein biosynthesis , microbiology and biotechnology , gene , biochemistry
The translation in vitro of mRNA from pseudorabies virus infected cells was studied using systems derived from wheat germ and from rabbit reticulocyte. The mRNA was shown by molecular hybridization to contain sequences complementary to virus DNA. Products of in vitro translation co-migrating with virus proteins on polyacrylamide gel electropherograms were detected and the major immune precipitation. Optimum conditions for the stimulation of amino acid incorporation in vitro were determined and found to be similar for mRNA from both infected and mock-infected cells.