Open AccessProteus mirabilis Phages 5006M, 5006M HFT k and 5006M HFT ak: Physical Comparison of Genome Characteristics
Author(s) -
G. H. J. Pretorius,
W. F. Coetzee
Publication year - 1980
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-49-1-33
Subject(s) - prophage , biology , proteus mirabilis , kanamycin , amp resistance , heteroduplex , genome , proteus , genetics , dna , strain (injury) , lysogenic cycle , bacteriophage , virology , microbiology and biotechnology , gene , escherichia coli , plasmid , anatomy
The genomes of proteus mirabilis phages 5006M, kanamycin resistance transducing variant 5006M HFT k and kanamycin-ampicillin resistance transducing variant 5006M HFT ak have been compared. Homo- and heteroduplex and partial denaturation mapping analyses were performed. The results confirm a sequential headful packaging mechanism, facilitate mapping of the ampicillin resistance marker, demonstrate a hairpin loop structure in both variants, reveal a common insertion site for 8 X 10(6) mol. wt. non-phage DNA in both variants and implicate a role for the non-inducible cryptic host strain prophage 5006M in the generatin cycle of the variant phages.