Open AccessSindbis Virus RNA Replication. II. Strand Composition and Metabolic Fate of the Multi-stranded RNA Species
Author(s) -
Christine W. Czarniecki,
T. Sreevalsan
Publication year - 1980
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-48-1-75
Subject(s) - biology , sindbis virus , rna , virology , viral replication , composition (language) , virus , alphavirus , rna dependent rna polymerase , genetics , gene , linguistics , philosophy
Double-stranded RNA from SB virus-infected cells was denatured and analysed on agarose-methylmercuric hydroxide gels. Equimolar amounts of three single-stranded species with mol. wt. of 4 x 10(6), 2.5 x 10(6) and 1.8 x 10(6) were found. Pulse and chase experiments in infected cells established a precursor-product relationship between the multi-stranded and single-stranded virus RNA species. The present results support the model in which the 49S and 26S species of virus RNA are synthesized in infected cells from two distinct replicating structures.