Open AccessCatalysis of Adenovirus DNA Synthesis in vitro by DNA Polymerase
Author(s) -
Charles R. Shaw,
David Rekosh,
W. C. Russell
Publication year - 1980
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-48-1-231
Subject(s) - biology , virology , dna polymerase , polymerase , dna , in vitro , dna polymerase ii , dna synthesis , microbiology and biotechnology , polymerase chain reaction , genetics , gene , reverse transcriptase
DNA replication complexes were purified from adenovirus type 5 (Ad5)-infected HeLa cells. DNA synthesis by these complexes in vitro was extremely sensitive to the inhibitors dideoxythymidine triphosphate, N-ethyl maleimide and p-hydroxymercuribenzoate. The bound DNA polymerase was released from the complexes by limited digestion with micrococcal nuclease. This released polymerase preferred poly(rA):(dT)12-18 as template over activated calf thymus DNA. These results are compatible with the major polymerase in the replication complex being of the gamma class.