Open AccessVirus-specific DNA Sequences Present in Cells which Carry the Herpes Simplex Virus Thymidine Kinase Gene
Author(s) -
A. C. Minson,
G. Darby,
P. Wildy
Publication year - 1979
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-45-2-489
Subject(s) - biology , thymidine kinase , herpes simplex virus , gene , microbiology and biotechnology , ecori , virology , dna , transformation (genetics) , restriction enzyme , nucleic acid sequence , thymidine , virus , genetics
Two independently derived cell lines which carry the herpes simplex type 2 thymidine kinase gene have been examined for the presence of HSV-2-specific DNA sequences. Both cell lines contained 1 to 3 copies per cell of a sequence lying within map co-ordinates 0.2 to 0.4 of the HSV-2 genome. Revertant cells, which contained no detectable thymidine kinase, did not contain this DNA sequence. The failure of EcoR1-restricted HSV-2 DNA to act as a donor of the thymidine kinase gene in transformation experiments suggests that the gene lies close to the EcoR1 restriction site within this sequence at a map position of approx. 0.3. The HSV-2 kinase gene is therefore approximately co-linear with the HSV-1 gene.