Open AccessIn vitro Packaging of Exogenous DNA by Salmonella Phage P22
Author(s) -
Elisabeth Strobel,
Horst Schmieger
Publication year - 1979
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-45-2-291
Subject(s) - biology , dna , in vitro , salmonella , virology , enzyme , dna synthesis , microbiology and biotechnology , genetics , biochemistry , bacteria
We describe in vitro conditions for packaging of exogenous DNA of Salmonella phage P22 which has terminally redundant, circularly permuted DNA. The method is a modification of the Kaiser-Masuda procedure. The most important aspect is to prepare all components (proheads, enzymes and concatemeric DNA) in end- cells. The influence of several factors such as DNA- and Mg2+ concentration and kinetics has been investigated.