Open AccessThe Sensitivity of Measles Virus Haemolysin to Acetone and the Preparation of Mono-specific Human Anti-Haemolysin by Absorption
Author(s) -
Mervyn Armstrong,
Kenneth Fraser,
P. V. Shirodaria
Publication year - 1979
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-44-2-541
Subject(s) - measles virus , measles , hemolysin , virology , biology , antibody , virus , acetone , neutralization , staining , absorption (acoustics) , microbiology and biotechnology , immunology , virulence , vaccination , biochemistry , materials science , genetics , gene , composite material
The haemolysin of measles virus, either in the virion or in infected cells, is functionally and antigenically sensitive to acetone. Absorption of human sera with acetone-treated, measles virus-infected cells removes antibodies to all measles virus structural antigens except haemolysin. The antibody titres of absorbed sera give good correlation in HLI, neutralization and fluorescent antibody staining on unfixed infected cells.