Open AccessRotavirus Polypeptides
Author(s) -
Margaret E. Thouless
Publication year - 1979
Publication title -
journal of general virology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-44-1-187
Subject(s) - rotavirus , biology , methionine , polyacrylamide gel electrophoresis , gel electrophoresis , microbiology and biotechnology , virology , virus , lysis , biochemistry , amino acid , enzyme
Rotavirus infected monkey kidney cells (LLC-MK2) have been labelled with 35S-methionine in the presence of actinomycin D. The cells have been lysed with SDS and the polypeptides separated by discontinuous polyacrylamide gel electrophoresis. Rotavirus polypeptides began to appear 4 to 5 h p.i.; incorporation was maximum at 8 h, but all the polypeptides were still being made 15 to 18 h p.i. Tissue culture adapted calf rotavirus particles were labelled with 35S-methionine and the polypeptides compared with cell associated rotavirus polypeptides. There were four inner coat, four outer coat and three non-structural polypeptides. Several of the outer coat polypeptides have altered mol. wt. on maturation. The polypeptides of rotavirus from seven species (human, pig, calf, lamb, mouse, foal and rabbit) have been compared and their mol. wt. calculated. The polypeptides fell into the same relative groupings for each virus, but there were variations in the mol. wt. of most comparable polypeptides. The polypeptides of tissue culture adapted and non-adapted calf rotavirus from the same original isolate varied only in one of the non-structural polypeptides.