Open AccessThe Effect of Inhibitors of Glycosylation on Interferon Production in Human Lymphoblastoid Cells
Author(s) -
B. Cundliffe,
John Morser
Publication year - 1979
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-43-2-457
Subject(s) - lymphoblast , glucosamine , glycosylation , biology , interferon , protein biosynthesis , virology , cell culture , rna , microbiology and biotechnology , biochemistry , gene , genetics
Interferon production was inhibited in the Namalwa line of human lymphoblastoid cells by treatment with 2-deoxy-D-glucose or D-glucosamine. D-Glucosamine also inhibited protein synthesis and the cells were no longer viable, whereas 2-deoxy-D-glucose allowed protein and RNA synthesis to continue at control rates, and the cells remained fully viable. It is concluded that a glycosylation step is essential for production of lymphoblastoid interferon.