Open AccessCyclic Expression of Antigen and Infectious Virus in a BHK Cell Line (0-853) Persistently Infected with an SSPE Strain of Measles Virus
Author(s) -
Natalie E. Cremer,
Lyndon S. Oshiro,
Shirley J. Hagens
Publication year - 1979
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-42-3-637
Subject(s) - subacute sclerosing panencephalitis , virology , measles virus , biology , virus , antigen , baby hamster kidney cell , measles , strain (injury) , immunology , vaccination , anatomy
Establishment and characteristics of a baby hamster kidney cell line (BHK 0-853) persistently infected with a subacute sclerosing panencephalitis (SSPE) strain of measles virus (Lec strain) is described. The persistent infection was easily and repeatedly established and no special conditions were required. There was a predictable fluctuation in expression of virus intracellular and membrane antigens which varied from greater than 90% to less than 1% of the cells demonstrating these antigens during the first 6 or 7 passages. Thereafter, fluctuation of antigen and infectious virus expression continued in an unpredictable fashion.