Open AccessTransformation of Rat Cells by the Hybrid Virus Ad22+ HEY
Author(s) -
Denise A. Galloway,
Eugene Lukanidin,
William C. Topp,
Joe Sambrook
Publication year - 1979
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-42-2-339
Subject(s) - biology , virology , transformation (genetics) , cell culture , virus , genome , adenoviridae , dna , antigen , microbiology and biotechnology , cell fusion , sv40 large t antigen , gene , genetics , recombinant dna , transfection
A set of four isogenic rat cell lines transformed by Ad22+ HEY have been studied. While all of the cell lines synthesize SV40 T antigen, only one expresses adenovirus 2 T antigen: none expresses SV40 V antigen or adenovirus 2 fibre antigen. Three cell lines contain 1 to 2 virus equivalents of SV40 and adenoviral sequences per diploid quantity of rate cell DNA and the fourth line contains five copies of SV40 and 20 copies of the adenovirus genome. At least three of the cell lines contain DNA sequences from the helper adenovirus 2 in addition to sequences from the Ad2+ HEY genome. The patterns of integrated virus sequences are complex suggesting multiple insertions of both adenovirus and SV40 DNA sequences. SV40 can be rescued from three cell lines by fusion with permissive cells.