Open AccessPresence of a Covalently Linked Protein on Calicivirus RNA
Author(s) -
J. N. Burroughs,
F. Brown
Publication year - 1978
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-41-2-443
Subject(s) - infectivity , rna , biology , covalent bond , formamide , polyacrylamide gel electrophoresis , proteinase k , virology , urea , ribosome , microbiology and biotechnology , biochemistry , virus , enzyme , chemistry , organic chemistry , genetics , gene
The infective RNA of the calicivirus, vesicular exanthema virus, has been shown to contain a protein which is apparently linked to the RNA by a covalent bond. The protein remained bound to the RNA after boiling with SDS-mercaptoethanol-urea or treating with formamide-dimethylsulphoxide but was removed by incubating with proteinase K. The mol. wt. of the protein was estimated to be about 1o X 1O(3) by electrophoresis in highly cross-linked polyacrylamide gels. The infectivity of the RNA was destroyed by removal of the protein with proteinase K.