Open AccessComparison of Interferon Action in Interferon Resistant and Sensitive L1210 Cells
Author(s) -
Pierre-Yves Vandenbussche,
Bernard Lebleu,
John Wérenne
Publication year - 1978
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-41-1-161
Subject(s) - interferon , phosphoprotein , aminoacylation , biology , l1210 cells , cell , stimulation , protein biosynthesis , phosphorylation , microbiology and biotechnology , cell free system , rna , virology , translation (biology) , embryo , transfer rna , in vitro , biochemistry , messenger rna , cytotoxicity , endocrinology , gene
Translation inhibition, leu-tRNA aminoacylation and double-stranded RNA and ATP dependent phosphorylation were examined in interferon-treated and control cell-free lysates of leukaemic mouse L 1210 R and L 1210 S cells. No differences were observed between the respective interferon-treated and control cell-free extracts, except for the presence of an enhanced 67K dalton phosphoprotein fraction in interferon-treated L 1210 S cell-free extracts. In non-responding cell-free lysates, the lack of stimulation of a 67K dalton phosphoprotein fraction cannot be explained by the presence of an increased level of some inhibitory activity, such as a phosphatase.