Open AccessThe Multiplication of an Influenza C Virus in an Established Line of Canine Kidney (MDCK) Cells
Author(s) -
Kuniaki Nerome,
Masatoshi Ishida
Publication year - 1978
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-39-1-179
Subject(s) - biology , virology , trypsin , virus , inoculation , microbiology and biotechnology , virus quantification , cell culture , agar , tissue culture , orthomyxoviridae , influenza a virus , bacteria , immunology , in vitro , biochemistry , genetics , enzyme
JJ/50 and four other strains of influenza C virus grew in an established line of canine kidney (MDCK) cells. Multicycle virus growth was markedly enhanced by the addition of trypsin to the culture medium and these viruses could be passaged serially in this system. The addition of appropriate concentrations of trypsin to the agar overlay medium enabled plaquing of influenza C/JJ/50 virus. Titration by plaque assay on MDCK cells was more sensitive than that by intra-amniotic inoculation of fertile hens' eggs.