Open AccessRepeated 'Superinduction' of Interferon in Human Diploid Fibroblast Cultures
Author(s) -
Marzenna Wiranowska-Stewart,
T Chudzio,
William E. Stewart
Publication year - 1977
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-37-1-221
Subject(s) - biology , virology , ploidy , fibroblast , interferon , wi 38 , cell culture , genetics , gene
Human diploid fibroblast cultures induced to make interferon by the combination of polyriboinosinic acid-polyribocytidylic adic, cycloheximide and actinomycin D degenerate thereafter, owing to the irreversible nature of the inhibition induced by actinomycin D. However, cultures superinduced with the DNA-dependent RNA synthesis inhibitor 5,6-dichloro-I-beta-D-ribofuranosylbenzimadazole (DRB) survive, owing to the reversible nature of the inhibition induced by DRB, and can again be superinduced on several occasions.