Open AccessSimian Virus 40 -- Chinese Hamster Kidney Cell Interaction. IV. Enhanced Virus Replication in Infected Cells upon Treatment with Mitomycin C
Author(s) -
Christian Lavialle,
A. G. Morris,
H. G. Suárez,
S. Estrade,
J. Stévenet,
R. Cassingéna
Publication year - 1977
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-36-1-137
Subject(s) - biology , chinese hamster , mitomycin c , virus , virology , hamster , baby hamster kidney cell , permissiveness , cell culture , viral replication , kidney , microbiology and biotechnology , antigen , immunology , genetics
Chinese hamster kidney cells are semi-permissive to simian virus 40 (SV40). Exposure to mitomycin C (MC) of Chinese hamster kidney cells infected with SV40 DNA enhanced the yield of infectious virus 10- to 100-fold. This stimulation occurred whether the treatment was performed before or after infection. A simultaneous increase in the number of V antigen-synthesizing cells and virus-producing cells, as well as the virus burst size, was observed upon MC pretreatment, whereas the proportion of T antigen-synthesizing cells remained unchanged. MC pretreatment clearly stimulated virus DNA replication in SV40 virus-infected cells. Cells treated with MC exhibited an unbalanced growth pattern, with continuing protein synthesis in the absence of cell division and a markedly reduced ability to replicate the cellular DNA. These results suggest that MC enhances the permissiveness of Chinese hamster kidney cells by inducing the synthesis of a specific cellular factor(s) required for SV40 reproduction in Chinese hamster kidney cells.