Open AccessSynthesis of Bacteriophage 6 Double-stranded Ribonucleic Acid
Author(s) -
David L. Coplin,
James L. Van Etten,
Anne K. Vidaver
Publication year - 1976
Publication title -
journal of general virology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-33-3-509
Subject(s) - biology , rna silencing , bacteriophage , virology , chloramphenicol , rna , uracil , nucleic acid , microbiology and biotechnology , protein biosynthesis , virus , antibiotics , dna , escherichia coli , rna interference , biochemistry , gene
Uracil was incorporated into all three bacteriophage phi6 dsRNA segments throughout the infection cycle; the rates of incorporation into each of the three segments were approx. constant for the first 15 to 20 min and then increased rapidly until 50 min after infection. The medium and small dsRNA segments were produced in greater amounts than the large dsRNA segment at all times in the infection cycle. Inhibition of host RNA and protein synthesis with rifampin and chloramphenicol revealed that virus dsRNA synthesis immediately after infection was independent of either host function.