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Polyamines in Bacteriophage W-14 and in W-14-infected Pseudomonas acidovorans
Author(s) -
Anita Quail,
E. Karrer,
R. A. J. Warren
Publication year - 1976
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-33-1-135
Subject(s) - biology , bacteriophage , virology , microbiology and biotechnology , pseudomonas , bacteria , escherichia coli , genetics , gene
Bacteriophage phi W-14 is sensitive to osmotic shock. It contain sufficient free putrescine, 2-hydroxyputrescine and spermidine to neutralize about 15% of the DNA phosphates. The alpha-putrescinylthymine residues of the DNA could neutralize a further 25% of the phosphates. Label is transferred from ornithine to the alpha-putrescinyl residues of phi W-14 DNA. The rates of polyamine synthesis in Pseudomonas acidovorans are increased by phi W-14 infection.

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