Open AccessAnalysis of Baby Hamster Kidney Cells Persistently Infected with Lymphocytic Choriomeningitis Virus
Author(s) -
Trevor L. Stanwick,
B. E. Kirk
Publication year - 1976
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-32-3-361
Subject(s) - lymphocytic choriomeningitis , virology , biology , virus , hamster , baby hamster kidney cell , cell culture , kidney , microbiology and biotechnology , immunology , immune system , genetics , cd8
Baby hamster kidney cells were persistently infected with lymphocytic choriomeningitis (LCM) virus (BHKpi cells). After 21 passages of the BHKpi cells infectious virus could no longer be detected; however, the cultures continued to produce LCM virus particles which interfered with the replication of infectious LCM virus in BHKpi cells and protected mice from a subsequent intracranial inoculation of infectious LCM virus. Cultures of BHKpi cells appeared to consist of three cell populations: uninfected cells, infected cells containing infectious LCM virus, and infected cells releasing interfering particles of LCM virus.