Open AccessThe Use of Tris-HCl Buffer for Inoculation of Tomato Protoplasts with Tobacco Mosaic Virus
Author(s) -
Fusao Motoyoshi,
Nobuyuki Oshima
Publication year - 1976
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-32-2-311
Subject(s) - tris , inoculation , phosphate buffered saline , tobacco mosaic virus , protoplast , biology , virology , virus , buffer (optical fiber) , phosphate , lysine , horticulture , chromatography , chemistry , botany , microbiology and biotechnology , biochemistry , amino acid , telecommunications , computer science
When tris-HCL was used as the buffer for inoculation of tomato protoplasts with tobacco mosaic virus, a greater proportion became infected than when phosphate was used. Using 0-05 M-tris-HCl buffer, pH 8-0, in the presence of poly-L-ornithine or poly-D-lysine, 50 to 80 % infection was obtained.