Open AccessOccurrence of Polyamines in Coliphages T5, X174 and in Phage-infected Bacteria
Author(s) -
Uriel Bachrach,
Roland Fischer,
Ira B. Klein
Publication year - 1975
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-26-3-287
Subject(s) - putrescine , polyamine , spermidine , biology , bacteria , ornithine decarboxylase , spermine , biochemistry , dna , ornithine , microbiology and biotechnology , chromatography , enzyme , chemistry , arginine , genetics , amino acid
The polyamine spermidine and the diamine putrescine have been detected in coliphages T5 and phiX174. Polyamines were identified by thin-layer chromatography and mass-spectrometry of dansyl derivatives, as well as by ion-exchange chromatography. In phiX174 phages, polyamines were sufficient to neutralize 0.5% of DNA phosphates. The polyamine content of T5 phages depended on growth media and purification procedures, but at least 1% of DNA phosphates were neutralized by polyamines. After infection, an increase in cellular polyamine was noticed. This increase paralleled variations in ornithine decarboxylase activity.