Open AccessCharacteristics of a Bacillus megaterium Bacteriophage
Author(s) -
P. H. Cooney,
Robert J. K. Jacob,
Ralph A. Slepecky
Publication year - 1975
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-26-1-131
Subject(s) - bacillus megaterium , bacteriophage , lysis , biology , bacillus cereus , bacillus licheniformis , nucleic acid , microbiology and biotechnology , bacillus subtilis , dna , guanine , strain (injury) , cytosine , virology , lysogenic cycle , cereus , bacteria , biochemistry , escherichia coli , nucleotide , gene , genetics , anatomy
A bacteriophage which infects and lyses Bacillus megaterium ATCC 19213 was isolated from the soil. The phage produces lysis on nine strains of B, megaterium tested but did not lyse a Bacillus cereus or Bacillus licheniformis strain, nor any of eight Bacillus subtilis strains tested. Physical characteristics of the phage including morphology, size, thermal and pH stability, and buoyant density were examined. The nucleic acid is double-stranded DNA of mol. wt. 41.7 times 10 and 36 to 38.5 mol percent guanine plus cytosine (G+C).