Open AccessDetermination of the Molecular Weight of Bovine Enterovirus RNA by Nuclease Digestion
Author(s) -
D. Todd,
Samuel Martin
Publication year - 1975
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-26-1-121
Subject(s) - biology , rnase p , fractionation , rna , oligonucleotide , sephadex , nuclease , microbiology and biotechnology , urea , nucleotide , molecular mass , digestion (alchemy) , electrophoresis , gel permeation chromatography , chromatography , biochemistry , virology , dna , enzyme , chemistry , gene , organic chemistry , polymer
The mol. wt. of a [32P]-labelled bovine enterovirus RNA has been determined by digesting with pancreatic RNase and separating the resulting oligonucleotides using a two-stage fractionation method on DEAE-Sephadex in 7 M-urea at pH 7-6 and pH 3-0. We have estimated the number of nucleotides as 8612 plus or minus 55. This corresponds to a mol. wt. of 2-93 plus or minus 0-02 X 10-6 which is in aggrement with estimates obtained by sedimentation and gel electrophoresis techniques.