Open AccessTaxonomic Position of Aromatic-Degrading Denitrifying Pseudomonad Strains K 172 and KB 740 and Their Description as New Members of the Genera Thauera, as Thauera aromatica sp. nov., and Azoarcus, as Azoarcus evansii sp. nov., Respectively, Members of the Beta Subclass of the Proteobacteria
Author(s) -
HansJoachim Anders,
Anette Kaetzke,
Peter Kämpfer,
Wolfgang Ludwig,
Georg Fuchs
Publication year - 1995
Publication title -
international journal of systematic bacteriology
Language(s) - English
Resource type - Journals
eISSN - 1070-6259
pISSN - 0020-7713
DOI - 10.1099/00207713-45-2-327
Subject(s) - biology , 16s ribosomal rna , pseudomonas , strain (injury) , proteobacteria , microbiology and biotechnology , bacteria , genetics , anatomy
In the past workers have isolated several pseudomonad strains which have been used for studies of anaerobic aromatic metabolism. The best studied of these strains are strains KB 740T (T = type strain) and K 172T. The taxonomic positions of these two organisms were determined by classical methods, including experiments to determine substrate spectrum, quinone type, and total fatty acid composition. Our results clearly excluded these strains from the authentic genus Pseudomonas, which belongs to the gamma subclass of the Proteobacteria. Instead, the properties of these organisms indicated that they belong to the beta subclass of the Proteobacteria. The sequences of the 16S ribosomal DNA genes confirmed this conclusion and indicated that strain K 172T represents a new species of the genus Thauera, Thauera aromatica, and that strain KB 740T represents a new species of the genus Azoarcus, Azoarcus evansii.