Open AccessV-type H + -ATPase in the symbiosome membrane is a conserved mechanism for host control of photosynthesis in anthozoan photosymbioses
Author(s) -
Katie L. Barott,
Angus B. Thies,
Martín Tresguerres
Publication year - 2022
Publication title -
royal society open science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.84
H-Index - 51
ISSN - 2054-5703
DOI - 10.1098/rsos.211449
Subject(s) - biology , sea anemone , cnidaria , photosynthesis , scleractinia , algae , zooxanthellae , anthozoa , botany , symbiodinium , coral reef , symbiosis , ecology , coral , paleontology , bacteria
In reef-building corals (order Scleractinia) and giant clams (phylum Molluca), V-type H+ -ATPase (VHA) in host cells is part of a carbon concentrating mechanism (CCM) that regulates photosynthetic rates of their symbiotic algae. Here, we show that VHA plays a similar role in the sea anemoneAnemonia majano , a member of the order Actinaria and sister group to the Scleractinia, which in contrast to their colonial calcifying coral relatives is a solitary, soft-bodied taxa. Western blotting and immunofluorescence revealed that VHA was abundantly present in the host-derived symbiosome membrane surrounding the photosymbionts. Pharmacological inhibition of VHA activity in individual anemones resulted in an approximately 80% decrease of photosynthetic O2 production. These results extend the presence of a host-controlled VHA-dependent CCM to non-calcifying cnidarians of the order Actiniaria, suggesting it is widespread among photosymbiosis between aquatic invertebrates andSymbiodiniaceae algae.