
Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M 4 muscarinic receptors?
Author(s) -
MarisJohanna Tahk,
Jane Torp,
Mohammed A. S. Ali,
Dmytro Fishman,
Leopold Parts,
Lukas Grätz,
Christoph Müller,
Max Keller,
Santa Veikšina,
Tõnis Laasfeld,
Ago Rinken
Publication year - 2022
Publication title -
open biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.078
H-Index - 53
ISSN - 2046-2441
DOI - 10.1098/rsob.220019
Subject(s) - ligand (biochemistry) , fluorescence microscope , biology , receptor–ligand kinetics , fluorescence , receptor , biophysics , muscarinic acetylcholine receptor , affinities , drug discovery , g protein coupled receptor , binding site , fluorescence lifetime imaging microscopy , fluorescence anisotropy , ligand binding assay , biochemistry , physics , quantum mechanics , membrane