Open AccessLive imaging YAP signalling in mouse embryo development
Author(s) -
Bin Gu,
Brian Bradshaw,
Min Zhu,
Yu Sun,
Sevan Hopyan,
Janet Rossant
Publication year - 2022
Publication title -
open biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.078
H-Index - 53
ISSN - 2046-2441
DOI - 10.1098/rsob.210335
Subject(s) - biology , microbiology and biotechnology , live cell imaging , embryo , embryonic stem cell , regulator , signalling , mitosis , cell , genetics , gene
YAP protein is a critical regulator of mammalian embryonic development. By generating a near-infrared fusion YAP reporter mouse line, we have achieved high-resolution live imaging of YAP localization during mouse embryonic development. We have validated the reporter by demonstrating its predicted responses to blocking LATS kinase activity or blocking cell polarity. By time lapse imaging preimplantation embryos, we revealed a mitotic reset behaviour of YAP nuclear localization. We also demonstrated deep tissue live imaging in post-implantation embryos and revealed an intriguing nuclear YAP pattern in migrating cells. The YAP fusion reporter mice and imaging methods will open new opportunities for understanding dynamic YAP signallingin vivo in many different situations.