Open AccessMechanism of Hsp70 specialized interactions in protein translocation and the unfolded protein response
Author(s) -
Natacha Larburu,
Christopher J. Adams,
Chao-Sheng Chen,
Piotr R. Nowak,
Maruf M. U. Ali
Publication year - 2020
Publication title -
open biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.078
H-Index - 53
ISSN - 2046-2441
DOI - 10.1098/rsob.200089
Subject(s) - biology , hsp70 , mechanism (biology) , microbiology and biotechnology , heat shock protein , plasma protein binding , protein–protein interaction , computational biology , genetics , gene , philosophy , epistemology
Hsp70 chaperones interact with substrate proteins in a coordinated fashion that is regulated by nucleotides and enhanced by assisting cochaperones. There are numerous homologues and isoforms of Hsp70 that participate in a wide variety of cellular functions. This diversity can facilitate adaption or specialization based on particular biological activity and location within the cell. In this review, we highlight two specialized binding partner proteins, Tim44 and IRE1, that interact with Hsp70 at the membrane in order to serve their respective roles in protein translocation and unfolded protein response signalling. Recent mechanistic data suggest analogy in the way the two Hsp70 homologues (BiP and mtHsp70) can bind and release from IRE1 and Tim44 upon substrate engagement. These shared mechanistic features may underlie how Hsp70 interacts with specialized binding partners and may extend our understanding of the mechanistic repertoire that Hsp70 chaperones possess.