Open AccessTranscription factor assembly on the nicotinic receptor β4 subunit gene promoter
Author(s) -
Michael D. Scofield,
Lei BruschweilerLi,
Zhongming Mou,
Paul Gardner
Publication year - 2008
Publication title -
neuroreport/neuroreport
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.607
H-Index - 188
eISSN - 1473-558X
pISSN - 0959-4965
DOI - 10.1097/wnr.0b013e3282fbcef7
Subject(s) - biology , protein subunit , chromatin immunoprecipitation , transcription factor , interleukin 10 receptor, alpha subunit , nicotinic acetylcholine receptor , promoter , microbiology and biotechnology , nicotinic agonist , transcription (linguistics) , acetylcholine receptor , sp1 transcription factor , gene , gamma aminobutyric acid receptor subunit alpha 1 , gene expression , receptor , g alpha subunit , genetics , philosophy , linguistics
Nicotinic acetylcholine receptors are involved in a plethora of fundamental biological processes ranging from muscle contraction to formation of memories. The receptors are pentameric proteins whose subunits are encoded by distinct genes. Subunit composition of a mature nicotinic receptor is governed in part by the transcriptional regulation of each subunit gene. Here, using chromatin immunoprecipitation assays, we report the interaction of the transcription factors Sp1, Sp3, c-Jun and Sox10 with the beta4 subunit gene promoter in neuronal-like cell lines and rodent brain tissue. Our results corroborate previous in-vitro data demonstrating that these transcription factors interact with the beta4 promoter. Taken together, these data suggest that Sp1, Sp3, c-Jun and Sox10 regulate expression of the beta4 subunit gene in the mammalian brain.