Open AccessThe Choice of Enzyme for Human Pancreas Digestion Is a Critical Factor for Increasing the Success of Islet Isolation
Author(s) -
Meirigeng Qi,
Luis Valiente,
Brian McFadden,
Keiko Omori,
Shiela Bilbao,
Jemily Juan,
Jeffrey Rawson,
Stephen Scott,
Kevin Ferreri,
Yoko Mullen,
Mohamed El-Shahawy,
Donald C. Dafoe,
Fouad Kandeel,
Ismail H. Al-Abdullah
Publication year - 2015
Publication title -
transplantation direct
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.648
H-Index - 9
ISSN - 2373-8731
DOI - 10.1097/txd.0000000000000522
Subject(s) - collagenase , islet , medicine , endocrinology , transplantation , digestion (alchemy) , andrology , microbial collagenase , in vivo , biology , enzyme , diabetes mellitus , chemistry , biochemistry , chromatography , microbiology and biotechnology
We evaluated three commercially available enzymes for pancreatic digestion by comparing key parameters during the islet isolation process, as well as islet quality post-isolation.