Open AccessGeneration of Soluble Human Tumor Necrosis Factor-α Receptor 1-Fc Transgenic Pig
Author(s) -
Bumrae Cho,
Ok Jae Koo,
Jong Ik Hwang,
Hwajung Kim,
Eun Mi Lee,
Sunghoon Hurh,
Sol Ji Park,
Han Ro,
Jaeseok Yang,
Charles D. Surh,
Anthony J. F. d’Apice,
Byeong Chun Lee,
Curie Ahn
Publication year - 2011
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/tp.0b013e3182215e7e
Subject(s) - microbiology and biotechnology , tumor necrosis factor alpha , transgene , proinflammatory cytokine , chemokine , cell adhesion molecule , receptor , biology , chemistry , immunology , inflammation , biochemistry , gene
Acute humoral xenograft rejection (AHXR) is an important barrier to xenograft survival. Human tumor necrosis factor-α (hTNF-α) is one of the essential mediators of AHXR and induces activation of porcine endothelial cells (PECs), resulting in upregulation of major histocompatibility complex molecules, adhesion molecules, and proinflammatory chemokines. We investigated whether introduction of a soluble human tumor necrosis factor receptor I-Fc (shTNFRI-Fc) fusion gene can suppress activation of PECs and, more importantly, produced shTNFRI-Fc transgenic pigs.