Open AccessQuantitative Assessment of β-Cell Apoptosis and Cell Composition of Isolated, Undisrupted Human Islets by Laser Scanning Cytometry
Author(s) -
Ivan Todorov,
Indu Nair,
Alina Avakian-Mansoorian,
Jeffrey Rawson,
Keiko Omori,
Taihei Ito,
Luis Valiente,
Itzia Iglesias-Meza,
Chris Orr,
Keh Dong Shiang,
Kevin Ferreri,
Ismail H. Al-Abdullah,
Yoko Mullen,
Fouad Kandeel
Publication year - 2010
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/tp.0b013e3181f1db5d
Subject(s) - islet , flow cytometry , transplantation , apoptosis , biology , cell , pancreatic islets , dna fragmentation , in vivo , glucagon , fragmentation (computing) , microbiology and biotechnology , endocrinology , medicine , programmed cell death , insulin , biochemistry , ecology
Assays for assessing human islet cell quality, which provide results before transplantation, would be beneficial to improve the outcomes for islet transplantation therapy. Parameters such as percent β-cell apoptosis and cell composition are found to vary markedly between different islet preparations and may serve as markers of islet quality. We have developed fluorescence-based assays using laser scanning cytometry for assessing β-cell apoptosis and islet cell composition on serial sections of intact isolated islets.