Quantitative Assessment of β-Cell Apoptosis and Cell Composition of Isolated, Undisrupted Human Islets by Laser Scanning Cytometry | Zendy

Иван Тодоров | Zendy; Indu Nair | Zendy; Alina Avakian-Mansoorian | Zendy; Jeffrey Rawson | Zendy; Keiko Omori | Zendy; T. Ito | Zendy; Luís Prieto | Zendy; Itzia Iglesias-Meza | Zendy; Chris Orr | Zendy; Keh-Dong Shiang | Zendy; Kevin Ferreri | Zendy; Ismail H. Al-Abdullah | Zendy; Yoko Mullen | Zendy; Fouad Kandeel | Zendy

Open Access

Quantitative Assessment of β-Cell Apoptosis and Cell Composition of Isolated, Undisrupted Human Islets by Laser Scanning Cytometry

Author(s) -

Иван Тодоров,

Indu Nair,

Alina Avakian-Mansoorian,

Jeffrey Rawson,

Keiko Omori,

T. Ito,

Luís Prieto,

Itzia Iglesias-Meza,

Chris Orr,

Keh-Dong Shiang,

Kevin Ferreri,

Ismail H. Al-Abdullah,

Yoko Mullen,

Fouad Kandeel

Publication year - 2010

Publication title -

transplantation

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.45

H-Index - 204

eISSN - 1534-6080

pISSN - 0041-1337

DOI - 10.1097/tp.0b013e3181f1db5d

Subject(s) - islet , flow cytometry , transplantation , apoptosis , biology , cell , pancreatic islets , dna fragmentation , in vivo , glucagon , fragmentation (computing) , microbiology and biotechnology , endocrinology , medicine , programmed cell death , insulin , biochemistry , ecology

Assays for assessing human islet cell quality, which provide results before transplantation, would be beneficial to improve the outcomes for islet transplantation therapy. Parameters such as percent β-cell apoptosis and cell composition are found to vary markedly between different islet preparations and may serve as markers of islet quality. We have developed fluorescence-based assays using laser scanning cytometry for assessing β-cell apoptosis and islet cell composition on serial sections of intact isolated islets.

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