Open AccessDevelopment and Validation of a Multiplex, Bead-based Assay to Detect Antibodies Directed Against SARS-CoV-2 Proteins
Author(s) -
Robert A. Bray,
Jar How Lee,
P. Brescia,
Deepali Kumar,
Thoa g,
Remi Shih,
E. Steve Woodle,
Jonathan S. Maltzman,
Howard M. Gebel
Publication year - 2020
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/tp.0000000000003524
Subject(s) - multiplex , antibody , serology , antigen , covid-19 , population , computational biology , immunology , virology , biology , single domain antibody , medicine , bioinformatics , disease , pathology , environmental health , infectious disease (medical specialty)
Transplant recipients who develop COVID-19 may be at increased risk for morbidity and mortality. Determining the status of antibodies against SARS-CoV-2 in both candidates and recipients will be important to understand the epidemiology and clinical course of COVID-19 in this population. While there are multiple tests to detect antibodies to SARS-CoV-2, their performance is variable. Tests vary according to their platforms and the antigenic targets which make interpretation of the results challenging. Furthermore, for some assays, sensitivity and specificity are less than optimal. Additionally, currently available serological tests do not exclude the possibility that positive responses are due to cross reactive antibodies to community coronaviruses rather than SARS-CoV-2.