Open AccessA Feasible Method for Quantifying Living Pancreatic Human Islets in Murine Livers Posttransplantation by Confocal Laser Scanning Microscopy
Author(s) -
Feng Sui,
Wei Tang,
Johann Karunananthan,
Cindy Qi,
Jing Li,
Nicholas He,
Fouad Kandeel,
Junfeng Li
Publication year - 2020
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/tp.0000000000003191
Subject(s) - islet , transplantation , pancreatic islets , human liver , confocal , pancreas , biology , insulin , medicine , endocrinology , pathology , in vitro , biochemistry , geometry , mathematics
Current histological methods cannot accurately determine the survival rate of human pancreatic islets following portal vein infusion. This is due, in part, to the low number of infused islets relative to the whole liver. In this study, we assessed the ability of confocal laser scanning microscopy (CLSM) to track human islets posttransplantation.