Open AccessAntagomiR-199a Enhances the Liver Protective Effect of Hypoxia-Preconditioned BM-MSCs in a Rat Model of Reduced-Size Liver Transplantation
Author(s) -
Weichen Zhang,
Qi Chen,
Yufu Ye,
Bei Zou,
Yuanxing Liu,
Liqin Cheng,
Jun Yu,
Shusen Zheng
Publication year - 2020
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/tp.0000000000002928
Subject(s) - antagomir , proinflammatory cytokine , mesenchymal stem cell , transplantation , hypoxia (environmental) , vascular endothelial growth factor , neutralizing antibody , medicine , apoptosis , pharmacology , chemistry , immunology , antibody , microrna , pathology , inflammation , vegf receptors , biochemistry , gene , organic chemistry , oxygen
Reduced-size liver transplantation (LT) was invented to overcome the shortage of donor livers; however, it has proven to be more susceptible to ischemia-reperfusion injury. Bone marrow-derived mesenchymal stem cell infusion has been shown to be protective following LT. Optimization of MSC infusion has been performed, among which hypoxia preconditioning and miRNA modulation have shown promise. MiR-199a inhibition was reported to induce angioneogenesis; however, whether mir-199a inhibition enhances the protective effect of Bone marrow-derived mesenchymal stem cells in LT remains unknown. In this study, we combined antagomiR-199a with hypoxia-preconditioned MSC (H-MSC) infusion to discuss their effect and mechanism in a rat model of reduced-size LT.