Open AccessOptimizing Temperature and Oxygen Supports Long-term Culture of Human Islets
Author(s) -
Hirotake Komatsu,
Jeffrey Rawson,
Leonard Medrano,
Colin A. Cook,
Alyssa Barriga,
Nelson Gonzalez,
Mayra Salgado,
Keiko Omori,
Fouad Kandeel,
YuChong Tai,
Yoko Mullen
Publication year - 2019
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/tp.0000000000002280
Subject(s) - islet , transplantation , in vivo , andrology , diabetes mellitus , biology , endocrinology , chemistry , medicine , microbiology and biotechnology
Islet transplantation is a promising treatment for type-1 diabetes; however, donor shortage is a concern. Even when a pancreas is available, low islet yield limits the success of transplantation. Islet culture enables pooling of multiple low-yield isolations into an effective islet mass, but isolated islets rapidly deteriorate under conventional culture conditions. Oxygen (O2) depletion in the islet core, which leads to central necrosis and volume loss, is one of the major reasons for this deterioration.