Open AccessUse of Quantitative Real Time Polymerase Chain Reaction to Assess Gene Transcripts Associated With Antibody-Mediated Rejection of Kidney Transplants
Author(s) -
Kathy Dominy,
Candice Roufosse,
Hanneke de Kort,
Michelle Willicombe,
Paul Brookes,
Jacques Behmoaras,
Enrico Petretto,
Jack Galliford,
P. Choi,
David Taube,
H. Terence Cook,
A.G. McLean
Publication year - 2015
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/tp.0000000000000621
Subject(s) - polymerase chain reaction , real time polymerase chain reaction , gene , microarray , antibody , microarray analysis techniques , biology , immunology , gene expression , medicine , pathology , genetics
Microarray studies have shown elevated transcript levels of endothelial and natural killer (NK) cell-associated genes during antibody-mediated rejection (AMR) of the renal allograft. This study aimed to assess the use of quantitative real-time polymerase chain reaction as an alternative to microarray analysis on a subset of these elevated genes.