Open AccessA journey upstream: Fluctuating platelet-specific genes in cell-free plasma as proof-of-concept for using ribonucleic acid sequencing to improve understanding of postinjury platelet biology
Author(s) -
Lucy Z. Kornblith,
Cedric M.V. Bainton,
Alexander T. Fields,
Zachary A. Matthay,
Nina T. Magid,
Brenda NunezGarcia,
Arun Prakash,
Philip A. Kurien,
Rachael A. Callcut,
Mitchell J. Cohen,
Roland J. Bainton
Publication year - 2020
Publication title -
the journal of trauma and acute care surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.25
H-Index - 187
eISSN - 2163-0763
pISSN - 2163-0755
DOI - 10.1097/ta.0000000000002681
Subject(s) - rna , platelet , rna splicing , gene , biology , ex vivo , messenger rna , platelet activation , alternative splicing , gene expression , microbiology and biotechnology , in vivo , immunology , biochemistry , genetics
The mechanisms of aberrant circulating platelet behavior following injury remain unclear. Platelets retain megakaryocyte immature ribonucleic acid (RNA) splicing and protein synthesis machinery to alter their functions based on physiologic signals. We sought to identify fluctuating platelet-specific RNA transcripts in cell-free plasma (CFP) from traumatic brain injury (TBI) patients as proof-of-concept for using RNA sequencing to improve our understanding of postinjury platelet behavior. We hypothesized that we could identify differential expression of activated platelet-specific spliced RNA transcripts from CFP of patients with isolated severe fatal TBI (fTBI) compared with minimally injured trauma controls (t-controls), filtered by healthy control (h-control) data sets.