Open AccessMechanisms of the Beneficial Effect of Hypertonic Saline Solution in Acute Pancreatitis
Author(s) -
Ana Maria M. Coelho,
José Jukemura,
Sandra N. Sampietre,
Joilson O. Martins,
Nilza A. Molan,
Rosely Antunes Patzina,
Björn Lindkvist,
Sônia Jancar,
José Eduardo Monteiro da Cunha,
Luiz Augusto Carneiro D’Albuquerque,
Marcel Cerqueira César Machado
Publication year - 2010
Publication title -
shock
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 117
eISSN - 1540-0514
pISSN - 1073-2322
DOI - 10.1097/shk.0b013e3181defaa1
Subject(s) - acute pancreatitis , pancreatitis , myeloperoxidase , pancreas , medicine , hypertonic saline , nitric oxide synthase , endocrinology , inflammation , nitric oxide , trypsinogen , pancreatic disease , saline , chemistry , trypsin , enzyme , biochemistry
Administration of hypertonic saline (HS) solution to rats with acute pancreatitis (AP) decreases mortality and systemic inflammation. We hypothesized that these effects are related not only to systemic inflammatory reduction, but also to a reduction of the pancreatic lesion. Acute pancreatitis was induced in Wistar rats by injection of 2.5% sodium taurocholate. Animals were divided in groups: without AP, not treated AP, AP treated with NaCl 0.9%, and AP treated with NaCl 7.5%. Trypsinogen activation peptides and amylase activity were increased in ascitic fluid and serum and were not affected by treatment with HS. Pancreatic inflammation was evaluated by increased myeloperoxidase activity, malondialdehyde formation, and histopathology for severity of pancreatic lesions. The HS did not affect these parameters. Expression of cyclooxygenase 2 and inducible nitric oxide synthase was markedly increased in the pancreas of the AP group and was reduced by treatment with HS. This treatment also reduced the levels of TNF-α and IL-6 but not of IL-10 in the pancreatic tissue. These results show that HS modulates cytokine production and expression of enzymes responsible for inflammatory mediator production in the pancreas without affecting the severity of the pancreatic lesions.