Open AccessSingle-Cell RNA-seq of Human Myeloid-Derived Suppressor Cells in Late Sepsis Reveals Multiple Subsets With Unique Transcriptional Responses: A Pilot Study
Author(s) -
Dijoia B Darden,
Rhonda Bacher,
Todd M. Brusko,
Parker Knight,
Russell B. Hawkins,
Michael C. Cox,
Marvin Dirain,
Ricardo Ungaro,
Dina C. Nacionales,
Jaimar Rincón,
Marie-Pierre Gauthier,
Michael P. Kladde,
Azra Bïhorac,
Todd Brusko,
Frederick A. Moore,
Scott C. Brakenridge,
Alicia M. Mohr,
Lyle L. Moldawer,
Philip A. Efron
Publication year - 2020
Publication title -
shock
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 117
eISSN - 1540-0514
pISSN - 1073-2322
DOI - 10.1097/shk.0000000000001671
Subject(s) - sepsis , transcriptome , myeloid derived suppressor cell , immunology , immunosuppression , myeloid , peripheral blood mononuclear cell , suppressor , cancer , biology , medicine , gene , gene expression , genetics , in vitro
Increased circulating myeloid-derived suppressor cells (MDSCs) are independently associated with poor long-term clinical outcomes in sepsis. Studies implicate subsets of MDSCs having unique roles in lymphocyte suppression; however, characterization of these cells after sepsis remains incomplete. We performed a pilot study to determine the transcriptomic landscape in MDSC subsets in sepsis using single-cell RNAseq (scRNA-seq).